Each TOPO TA Cloning Kit contains linearized and topoisomerase I-activated pCR 2.1-TOPO Vector Salt Solution dNTPs Control Template and Primers M13 Forward and Reverse Primers One Shot Chemically Competent or Electrocomp E. coli S.O.C. Medium and a supercoiled control plasmid.The TOPO TA Cloning Kits for Sequencing provide a highly efficient 5 minute one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for sequencing.
Parental bacterial expression vector for TOPO TA cloning of PCR products.Home Resources Plasmid Files TA and GC Cloning Vectors pcDNA3.4-TOPO (linearized) pcDNA3.4-TOPO (linearized) Linearized mammalian vector with 3-T overhangs and bound topoisomerase for TOPO TA cloning of PCR products and high level expression.
Plasmid pcDNA3_3_TOPO_TA-ms2-VP64 from Dr. George Churchs lab contains the insert MS2-VP64 and is published in Nat Methods. 2016 May 23. doi 10.1038nmeth.3871. This plasmid is available through Addgene.For sticky end TOPO TA cloning Taq polymerase has a nontemplate-dependent terminal transferase activity that adds a single deoxyadenosine (A) to the 3-end of the PCR products. This characteristic is exploited in TOPO-cloning. For blunt end TOPO cloning the recipient vector does not have overhangs and blunt-ended DNA fragments can be cloned.