Plasmid pEGFP-C1 Lifeact-EGFP from Dr. Dyche Mullinss lab contains the insert Lifeact and is published in Bioarchitecture. 2015 Aug 281-14. Plasmid pEGFP-TRAIL from Dr. Bingliang Fangs lab contains the insert Tumor necrosis factor alpha related apoptosis inducing ligand and is published in Cancer Res. 2001 Apr 15. 61(8)3330-8. This plasmid is available through Addgene.Plasmid pCP20 problem - (reply 1) Creating empty vector - (reply 5) cloning vector and restriction mapping - (reply 2) software to replace Vector NTI and a simple cloning problem - (reply 3) Stable transfection problem - Problem with pBM vector and Phoenix packaging cells (reply 3) plasmid - (reply 2) no expression in pET 21 vector -
TurboGFP Control vector (SHC003 SHC003V SHC003H)This vector is a useful control for measuring transfection efficiency and optimizing shRNA delivery. The TurboGFP Control vector consists of the lentiviral backbone vector pLKO.1-puro containing a gene encoding TurboGFP driven by the CMV promoter.2018. Ramani K. et al. (2018). Role of A-Kinase Anchoring Protein Phosphorylation in Alcohol-Induced Liver Injury and Hepatic Stellate Cell Activation.The Monarch Plasmid Miniprep Kit is a rapid and reliable method for the purification of high quality plasmid DNA. This method employs standard cell resuspension alkaline lysis and neutralization steps with the additional benefit of color indicators at certain steps to easily monitor completion.