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Pmon Vector

This post categorized under Vector and posted on November 8th, 2019.

Plasmid pMON-HsNMT1 from Dr. Richard Kahns lab contains the insert HsNMT1 and is published in Methods Enzymol. 2002344186-93. This plasmid is available through Addgene.Plasmid pMON-HsNMT2 from Dr. Richard Kahns lab contains the insert HsNMT2 and is published in Methods Enzymol. 2002344186-93. This plasmid is available through Addgene.Vector Database. Digital collection of empty plasmid backbones from publications and commercially available sources . Help Center . Ordering & MTAs

The mor gene is expressed from vector sequences by readthrough from the nptII promoter. Growth at 42C inactivates the temperature sensitive Mu repressor (cts) and allows expression of the insert as well as genes controlling replication and integration of phage Mu. Induction of replication may increase copy number up to 50-fold. For plasmid replication Mu lysogens such as E.coli MH3870 are PMON source from 2001 modifyed by Algorithmics is available as pmonsrc-20011116.tar.gz. It contains support for several boards R4000 -style exceptions and uses the GNU make system. There is a PMON-VR fork for the NEC_VR4100 as part of the Agenda VR3 HPC project.

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